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Lab Protocols

The Immune Tolerance Network (ITN) provides the standard operating procedures and protocols, developed by ITN personnel and collaborators, free to use by the research community. Please note that no technical support is available. All works are licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 2.5 License.

Cell and Tissue Processing

ITN PBMC Processing SOP

Isolation and Cryopreservation of PBMC from whole blood

Collection and Cryopreservation of Lymphocytes from Lymph Node or Splenocytes from Spleen

KC-SOP-001

Collection and Preservation of Kidney or Liver Transplant Biopsy for ITN Study

v2.0

ITN091AI VIBRANT

On-site PBMC Isolation Using SepMate Tubes Standard Operating Procedure

ITN093AI DARE-APS

Preparation of Platelet-Poor Plasma from Sodium Citrate Tubes Standard Operating Procedure

ITN091AI VIBRANT

Renal Biopsy Processing for Single Cell Analyses Standard Operating Procedure

ITN091AI VIBRANT

Urine Processing for Single Cell Analysis and Proteomics Standard Operating Procedure

ITN093AI DARE-APS

Whole Blood Preservation with Smart Tubes Standard Operating Procedure

Flow Cytometry

ITN Flow Cytometry SOP

Microbiome

ITN079AD ACTIVATE

Microbiome Viability Assessment

Tissue Staining

46206.911

C4D Abcam - Paraffin AEC - Manual

v1.1

46206.909

C4D Frozen-FITC (1 Day Protocol)

v1.2

46206.78

H/E Frozen Protocol

v2.0

46206.871

H/E Paraffin Protocol

v3.0

46206.17

v2.0

46206.1017

Periodic Acid Methenamine Silver (PAMS)

v2.0

46206.52

Richard Allan MST

v2.0

46206.18

Rowley Masson Trichrome

v2.0

Type 1 Diabetes Real Time Tests

Diabetes Diagnostic Lab- C-peptide II (C-PEPII) Assay Standard

v7.0

Diabetes Diagnostic Laboratory - Glucose Testing Using the Roche Cobas c311

v4.0

GAD65Ab

Human Recombinant GAD-65 in Vitro Translation & Autoantibody Immuno-Radioassay

IA-2Ab

Human Recombinant IA-2 in Vitro Translation & Autoantibody Immuno-Radioassay

mIAA

Human Recombinant Insulin Autoantibody Immuno-Radioassay

ZnT8Ab

Human Recombinant ZnT8 in Vitro Translation & Autoantibody Immuno-Radioassay

Viral Load Testing

Cytomegalovirus DNA Detection and Quantification Protocol by Light Cycler-PCR with Hybridization Probes

Epstein-Barr Virus DNA Detection and Quantification Protocol by Light Cycler-PCR with Roche Primers and Probes Kit